Thanks for the help there Lighting-guy77,
I ended up buying a Pi 3B+ rather than using my Pi 3B, still wasn't working. My problem ended up being a bad LED at the start which in turn caused issues with the rest of the strip, I found this out by using a different segment and soldering new cables to it.
Now that my lights are syncing, I now have another issue that has arised:
All preset colour modes look great, however when I have HyperBian set to Capture Card my LED's are dull in colour. When I have bright red on the tv that Capture Card preview in HyperBian sees it as almost a brown colour and therefore the LED's are an off white. This happens when every colour is set, I cannot get any bright vibrant colours when I have it set to Capture Card.