I currently have Hyperion setup and controlling led lights on my PC.

Can I use this same Hyperion instance to recieve another video signal and output it to a different device?

So i'd have the following:

Hyperion PC Screen Grabber input > Adalight USB output

Hyperion Android Screen Grabber input > WLED output

Can this be done with a single PC or raspberry PI, or does each solution need its own controller?

How? Can you explain or link me a guide for this? Can't find anything on the internet.

Can i run multiple instances of Hyperion and control two ambilight systems with only one raspberry pi?

I have two TVs, each has its own leds and screen grabber. I want to control them both with a single computer or raspberry pi

I have the exact same question. Can I have two different TV ambilight setups controlled with Hyperion on a single raspberry pi?